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Sivaselvam, S. N.
- Chromosomal Characterization of Umblachery Breed of Cattle (Bos indicus) - a Famous South Indian Breed of Tamilnadu, India
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PDF Views:79
Authors
Affiliations
1 Department of Animal Genetics and Breeding, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai, Tamilnadu, IN
1 Department of Animal Genetics and Breeding, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai, Tamilnadu, IN
Source
Indian Journal of Science and Technology, Vol 1, No 6 (2008), Pagination: 1-3Abstract
Umblachery breed is an excellent draught cattle of Tamilnadu, noted for its strength and sturdiness especially used in the marshy fields for wed ploughing. Several genetic markers have been used for identification of farm animals. The present study was undertaken to characterize the Umblachery breed with chromosomes. The diploid chromosome number in Umblachery cattle was 2n=60 comprising of 29 pairs of autosomes and one pair of sex chromosomes. All autosomes are acrocentric, the X- chromosome is submetacentric and Y chromosome is acrocentric. C-banding in Umblachery cattle was showed all autosomes with darkly stained centromere but the sex chromosomes were found to be C-band negative.Keywords
Chromosomal Characterization, Umblachery CattleReferences
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- Molecular Typing and Mapping of MHC Class II-DRB3 Gene in Indian River Buffaloes (Bubalus bubalis)
Abstract Views :375 |
PDF Views:103
Authors
Affiliations
1 Dept. of Animal Genetics and Breeding, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Chennai - 600 007, IN
1 Dept. of Animal Genetics and Breeding, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Chennai - 600 007, IN
Source
Indian Journal of Science and Technology, Vol 3, No 5 (2010), Pagination: 557-560Abstract
The present study was undertaken with the objectives of characterizing Bubu-MHC loci by PCR and genotyping MHC loci for allelic variation. The PCR product of second exon of the Bubu-MHC-DRB3 gene (304 bp) exhibited genetic polymorphism while digesting with HaeIII enzyme resulting in three restriction fragment patterns in Murrah, four in Surti and three in Murrah graded buffaloes. In all the three genetic groups, the pattern `b' (82, 222 bp) was frequently observed. The restriction fragment analysis with RsaI revealed five patterns in Murrah and three in Surti. The pattern `s' (67, 93, 144 bp) with a frequency of 0.4444 and `l' (67, 237 bp) with a frequency of 0.5000 were observed. Microsatellite typing revealed nine alleles ranging from 160 to 212 bp at the second intron. In Murrah, the allele 190 bp was observed exclusively. In Surti, alleles 192 and 212 bp were observed more frequently. RsaI enzyme revealed more polymorphic patterns of DRB3 than HaeIII. Microsatellite typing provided certain breed-specific alleles. This gene was physically localized to chromosome 2p following tyramide signal amplification in between bands 15-22 using (cDNA probes derived from Bos taurus cattle) fluorescence in situ hybridization.Keywords
Bubu-MHC, PCR-RFLP, FISH, BuffaloesReferences
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- Microsatellite Analysis of Kangayam Cattle (Bos indicus) of Tamilnadu
Abstract Views :352 |
PDF Views:60
Authors
Affiliations
1 Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, IN
1 Dept. of Animal Genetics & Breeding, Madras Veterinary College, Chennai – 600 007, IN
Source
Indian Journal of Science and Technology, Vol 2, No 10 (2009), Pagination: 38-40Abstract
Assessment of genetic variability in Kangayam breed of cattle in Tamilnadu, South India was carried out using 25 bovine microsatellite markers. The mean number of alleles was 4.04 ± 0.09 with a range of 2 to 6 and the allele size ranged from 94 to 300 bp. The frequency distribution of alleles in the breed was from 0.0104 to 0.9167. The estimated heterozygosity was 0.6183 ± 0.01 and the PIC was 0.5628 ± 0.03. The overall mean within-population inbreeding estimate (FIS) value (- 0.084) suggested excess of heterozygotes in the population. In addition, higher PIC value indicated the scope for maintaining variation in the population and strategies to take meaningful conservation.Keywords
Cattle, Kangayam, Microsatellites, PICReferences
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